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Clinical significance of oxidative stress markers as angioinvasion and metastasis indicators in papillary thyroid cancer

In-gel digest and MS Coomassie-stained gel pieces were digested in gel with trypsin 57 , and desalted 58 peptide mixtures were analyzed on an Easy-nLC 1200 coupled to a Q Exactive HF mass spectrometer (both Thermo Fisher Scientific) as described previously 26 with slight modifications: peptide mixtures were separated using a 57-min and 87-min segmented gradient, respectively, of 10%33%50%90% of HPLC solvent B (80% acetonitrile in 0.1% formic acid) in HPLC solvent A (0.1% formic acid) at a flow rate of 200 nl min 1

Clinical safety: Use only by the current labelled topical route