INTRODUCTION Methotrexate (MTX) is a synthetic antimetabolite with many therapeutic indications, mainly anti-inflammatory and immunosuppressant at relatively low doses and antineoplastic at high doses.[ , ] MTX competitively inhibits dihydrofolate reductase, so decreasing tetrahydrofolate level leads to blocks the synthesis of purines, pyrimidines, DNA, ribonucleic acid, thymidylate, and proteins.[ , , ] It is well known that MTX is a hepatotoxic drug, and this leads to limitation of its clinical use
This result eliminates the possibility that the survivors of the high temperature (42 O C) in the first experiment a are either heat-tolerant mutants or persistor-like cells that behave similarly to persistors of antibiotic treatments
Attwell, D., Mishra, A., Hall, C
10.1080/00325481.2019.1647080 2 American Psychiatric Association (2013)