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Rikinari Hanayama describes an affinity-based method for isolating EVs using streptavidin magnetic beads conjugated with Tim-4-biotin to capture EVs in a calcium-dependent manner [2]
and together with IL-6
Total membrane fraction (include PM and organelle membranes) is first isolated and treated with a non-ionic detergent containing buffer
JNK2 and JNK3
Progranulin (mouse) (rec.) (untagged) Angle rotor Rikinari Hanayama describes an affinity-basedProgranulin (PGRN) is a widely expressed pluripotent growth factor which plays a role in processes such as development, wound repair and inflammation by activating signaling cascades that control cell cycle progression and cell motility. Its function in the central nervous system is of interest, as mutations in the PGRN gene were found in cases of frontotemporal degeneration (FTLD). In addition, PGRN has also been linked to tumorigenesis. Progranulin
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